Avviso di Videoconferenza: Fethi Bedioui - "Surface functionalization of microchip materials by electrochemistry at the microscale for LAB-ON-CHIP Applications" - Edificio C11, Sala Consiglio (1° piano)

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Data evento
Data inizio evento: 
30/11/2015 - 15:30
Data fine evento: 
30/11/2015 - 16:30
Data pubblicazione evento
Pubblicato il: 
25/11/2015

Avviso di Videoconferenza

(DEPARTMENT OF MOLECULAR SCIENCE AND NANOSYSTEMS  - Università Ca' Foscari Venezia)

 

Prof. Fethi Bedioui
PSL Research University, Chimie ParisTech, Unité de Technologies
Chimiques et Biologiques pour la Santé, Paris, France.

 

SURFACE FUNCTIONALIZATION OF MICROCHIP MATERIALS BY ELECTROCHEMISTRY AT THE MICROSCALE FOR LAB-ON-CHIP APPLICATIONS

 

Abstract.


The development of micro analytical systems is of major interest for trace contaminant analysis, particularly for water emerging contaminants such as pharmaceutical residues (diclofenac for example). As a lab-on-chip integrates all steps of an analysis procedure in a single device, analysis can be performed in an automated format with reduced time and cost. In this context, we propose new analytical microfluidic devices for trace quantitation in complex matrices, using a single channel configuration. Our strategy consists of the immobilization of selective and original ligands (aptamers) in a well-defined and micrometric zone inside the microchannel for the target extraction and pre-concentration. This integrated pretreatment step will be followed inside the microdevice by electrokinetic separation and on-line detection. To this end, we developed an original two-step strategy combining the use of scanning electrochemical microscopy (SECM) and molecular chemistry, via a “click” reaction (copper(I)-catalyzed azide alkyne cycloaddition) to functionalize Dyneon THV microchannel. Dyneon THV is indeed a promising fluorinated polymeric material for microsystems. The first step consisted of the local reduction of the Dyneon using the SECM tip to activate the surface by the creation of a carbonized zone and notably the formation of surface alkyne functions. This was followed by direct click reaction with azide-tagged ligand for its immobilization. The dimensions of the zone may be controlled by substrate-tip distance, tip speed, and tip size. The proof of concept was demonstrated by stable functionalization of the microchannel with a fluorescent dye and also with a fluorescent aptamer with high affinity for diclofenac.

 

Ultimo aggiornamento: 25-11-2015 - 11:42
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